Proof that
DNA was the source of genetic information
  1952
A.D. Hershey and Martha Chase
(fig. 2)used radioisotope tagging of the protein coat of a virus
with 35SULPHUR and the DNA inside with 32PHOSPHOROUS.
After the virus replicated and killed the bacteria cells the P was
found in the bacteria and S in solution above after centrifugant.
Thus proving DNA was the source of genetic
information.
Structure
of DNA
Rosalind Franklin
(crystalographer)
X Ray diffraction (Fig. 1 )
revealed a helical pattern to
the structure of
DNA in 1953 the information was later used by
Watson and Crick to determine the structure of DNA. They never mentioned
her in their biography.
 
Chargaff -
(1951) noted "regularities"
in the base composition of nucleic acids, which they considered "reflected
the existence in all DNA preparations of certain structural principles"
. He identified a species-invariant component of the base composition, >
%A = %T and %C = %G.
This "first parity rule" was
dramatically confirmed by the Watson-Crick double-helix model (Watson and
Crick, 1953)
Crick and
Watson fig. 3
- deduced the
structure of DNA in 1953 using some information from Chargaff and
Franklin's works. They determined that DNA is a double helix made of ATCG
nucleotides 2nm in width with a helical turn every 3.4 nm.
They won the Nobel prize for this work in 1962.
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